System overview

  • Flexible platform for trapping and tracking of nanoparticles for measurements from single-molecules to entire cells
  • Compatible with inverted microscopes for combined experiments, e.g.,
    • Carl Zeiss Axiovert 200, Axio Observer
    • Nikon TE 2000 & Eclipse Ti/Ti2
    • Olympus IX70/71/73
    • Leica DMi8
  • Compatible with all major light microscopy techniques such as DIC and fluorescence
  • Spectral range of 400-900 nm for undisturbed fluorescence imaging
  • Optical components for trapping are optimized for 1064 nm wavelength
  • Automated calibration for enhanced productivity
  • All major components are motorized and computer-controlled
  • No safety goggles needed (Class 1 laser) and no alignment by hand
  • Optional external standard modules such as confocal units, CCD cameras, detectors

NanoTracker™ 2 head

  • Closed head design prevents stray light and airborne noise for noise-free measurements
  • Liquid-safe, robust and drift-minimized design for highest stability
  • Optional sample positioning piezo stage with up to 100 ×100 ×100 μm³ travel range and closed-loop control for accurate trap calibration, fast vertical scanning and experimental flexibility
  • Software controlled motorized precision stage with 20 ×20 mm² travel range
  • Oil or water immersion lenses for high power or low spherical aberration for advanced trapping applications
  • Four sensitive InGaAs photo detectors with up to 3.5 MHz bandwidth (16 bit sampling)
  • Decoupled detection of XY and Z for optimized Z sensitivity
  • Precise focus adjustment via software

NanoTracker™ 2 sample holder options

  • Standard slides, cover glasses or Petri dishes can be used
  • Multichannel LaminarFlowCell (LFC™) is a coverslip-based fluid cell with laminar flow perfusion capabilities for single-molecule applications
  • Living cell studies with temperature control (ambient to 60 °C), perfusion and gas flow (CO2) for 35 mm Petri dishes with glass bottom from Wilco, BD, and Corning

All fluid cells and temperature control options

NanoTracker™ 2 laser unit

  • Ultra-stable custom-designed laser (1064 nm)
  • < 0.5 % intensity stability
  • 3, 5 and 10 W laser power
  • Class 1 laser certification

NanoTracker™ 2 steering unit

  • True 3D continuous beam steering through the full field of view
  • One or two beams with adjustable power distribution
  • One beam with XY steering by advanced piezo mirrors or AOD, and Z steering
  • 500 μsec response time for steering unit

NanoTracker™ 2 controller

  • State-of-the-art controller with lowest noise levels
  • 60 MHz sample rate with 16 bit for XY and Z detection
  • Signal Access Module (SAM) with more than 20 input/output channels
  • TTL access and power supply for external equipment
  • High-speed Ethernet link and intelligent grounding concept for maximum bandwidth and performance

NanoTracker™ 2 software

  • Easy to use Java™-based user interface for intuitive instrument control
  • Point and Trap™ beam steering
  • Automated force versus displacement calibration
  • JPK‘s proven DirectOverlay™ functionality for precise matching of trap and sample position
  • Embedded camera control for high-end EM-CCD‘s
  • Advanced oscilloscope functionality and online measurement of distances, cross sections, and many more
  • Powerful force spectroscopy with the new RampDesigner™ for advanced force clamp or force ramp experiments
  • JPK’s ExperimentPlanner™ for easy design of advanced experimental routines
  • Advanced and high-speed batch processing force curve analysis with huge number of fitting models including WLC, FJC or step fitting algorithms
  • Complete environmental control via software
  • User-programmable software for advanced experiments
  • User-definable shortcut buttons
  • Traps, motor and shutter steering control via gamepad

Optional modular items for enhanced flexibility

  • Single-beam/dual-beam configuration
  • Detection unit: equipped as single-beam or dual-beam
  • AOD option for faster beam steering and optimized trap multiplexing
  • Closed-loop piezo stage configurations: 3-axes, 1-axis (only Z) or none
  • Axial trap control for independent focus of traps and microscope
  • Lasers with different output power
  • Choice of microscope objectives: magnification, immersion medium, TIRF
  • MagneticTwister™ add-on


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Instrument modes

  • 3D particle tracking
  • Point and Trap™ for easy trap positioning
  • Online calibration based on power spectrum
  • Advanced Force Spectroscopy including Force Clamp and Force Ramp with the new JPK RampDesigner™
  • Active and passive force mapping
  • Micro-rheology mode
  • Optical sorting
  • Trap oscillation
  • Multiplexing for 3D trap arrays
  • Line traps & circular traps
  • Microfluidics control
  • Nanoassembly
  • Optical z-stacking

Advanced optical microscopy modes

  • Brightfield transmission illumination (standard)
  • Differential Interference Contrast (DIC) in transmission (standard)
  • Epi-fluorescence microscopy (standard)
  • Raman spectroscopy
  • TIRF microscopy
  • Confocal microscopy
  • FRET microscopy and more

Applications

Single-molecules & biopolymers
  • Intra-molecular elasticity & protein folding dynamics
  • Motor protein tracking
  • DNA/RNA mechanics
  • Protein-DNA binding
  • Nanopores & 3D polymer network probing
Cell biology applications
  • Membrane organization (e.g., lipid rafts)
  • Trans-membrane processes, trafficking
  • Intracellular forces
  • Receptor-ligand experiments
  • Cell mechanics and cell motility
  • Membrane tether dynamics
  • Micro-rheology of cells and gels
Cell-particle interaction and infection studies
  • Tracking of pathogen-host interaction and escape forces
  • Bacterial and virus adhesion forces
  • Local gene or drug delivery
  • Entrance mechanism studies
  • Nanotoxicity & endocytosis studies
Advanced measurements
  • Complex optical trap geometries
  • Optical guiding & artificial crystal building
  • Local field enhancement & Raman/SERS applications
  • Brownian motion tracking, Photonic Force Microscopy (PFM)
  • Colloidal and polymer meshworks force probing
  • Video particle tracking and optical spectroscopy