CellHesion® 200 specifications
- Innovative platform for cell adhesion/cell mechanics research for measurements from single-molecules to entire cells and tissue
- Compatible with inverted microscopes for combined experiments, e.g., Zeiss Axio Observer, Axiovert A1, Nikon Ti/TE 2000, Nikon Ti2, Olympus IX series and Leica DMi series
- Compatible with all major light microscopy techniques such as DIC and phase contrast or fluorescence and confocal techniques such as TIRF, FRAP, LSM and others
- Compatible with upright macroscopes and stereo microscopes such as Zeiss AxioZoom V.16, Leica Z16 ApoA, Leica M205FA and Olympus MVX10
- Automated range adaption in z direction for strong corrugated surfaces
- Cantilever sensor lifting system with >110µm travel range with closed-loop control through high-speed capacitive sensor feedback
- Equipped with motorized precision stage, in particular for tissue probing, within 20×20mm range
- Living cell studies in native environment with tempeature control, perfusion and gas flow (CO2) on coverslips with the JPK BioCell™ or 35mm Petri dishes with or without glass bottom from Wilco, BD, WPI with the JPK PetriDishHeater™
- All parts which came in contact with the sample are easy to clean
- Compatible with standard incubators (specify model)
- High-throughput determination of cellular interaction parameters through JPK software batch processing
- Single-molecule events, work of removal, maximum adhesion forces, number of unbinding events and viscoelastic parameters such as Young’s modulus in one system
DOWNLOAD BROCHURELiterature
- Mechanosensing is critical for axon growth in the developing brain. Koser et al., Nature Neuroscience 19, 1592–1598 (2016)
- Testing the differential adhesion hypothesis across the epithelial−mesenchymal transition. Pawlizak et al., New J. Phys. 17, 083049 (2015)
- Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup. Gonnermann et al., Integr. Biol. (2015)
- Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines: An approach for evaluating cellular invasion. Omidvar et al., Journal of Biomechanics 47(13), 3373–3379 (2014)
- Auxetic nuclei in embryonic stem cells exiting pluripotency. Pagliara et al., Nature Materials 13, 638-644 (2014)
- Assay for characterizing the recovery of vertebrate cells for adhesion measurements by single-cell force spectroscopy. Schubert et al., FEBS 588(19), 3639-48 (2014)
- Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6. Te Riet et al., J. Cell Sci. 127(Pt 7), 1595–1606 (2014)
- Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy. Friedrichs et al., Nature Protocols 5(7), 1353-1361 (2010)