Optical Tweezers/3D Particle Tracking

Optical tweezers and optical trapping systems. Nanooptics in Life Sciences. Using nanoparticles as a local sensor or probe. (optical tweezers, opical trapping, opical trap, particle tracking, 3D particle tracking, particle detection, nanoparticles, nanooptics, JPK Instruments AG)

optical tweezers, opical trapping, opical trap, particle tracking, 3D particle tracking, particle detection, nanoparticles, nanooptics, JPK Instruments AGOptical tweezers and optical trapping systems. Nanooptics in Life Sciences. Using nanoparticles as a local sensor or probe.

Optical tweezers and optical trapping systems. Nanooptics in Life Sciences. Using nanoparticles as a local sensor or probe.

A whole new spectrum of applications

The unique combination of 3D positioning, detection, and manipulation provided by OT and the high-resolution imaging and surface property characterization of AFM opens up a whole new spectrum of applications such as cellular response, cell-cell or cell matrix interactions, immune response, infection or bacterial/virus/nanoparticle uptake processes, and more.

Single-molecule applications with up to 14 degrees of freedom
With a multitude of available handles, interaction, and detection sites, OT-AFM significantly extends the range of single-molecule applications.


Spectacular live cell applications with the OT-AFM system
Cellular response, cell-cell or cell matrix interactions, immune response, infection or bacterial/virus/nanoparticle uptake processes are just a few of the examples that can be investigated with JPK’s new state of the art OT-AFM platform. JPKs proven AFM and OT core technologies, combined with fluorescence microscopy, have set the ultimate benchmark for live cell applications.  

Triggering immune signaling that affects cell adhesion
Trigger cellular responses by using functionalized particles or modified microorganisms is a common method. The resulting  changes in cellular structure, dynamics, and mechanical properties can be investigated using AFM-based methods. Delivering objects to specific regions of interest on the cell, however, is very difficult to achieve. OT provides the perfect tool for manipulating the sample and triggering cellular response, at a well-defined (an exactly-defined?!) time and location. This significantly improves the throughput, flexibility, and reproducibility of these studies. In this application, the influence of signaling between dendritic cells (DCs) and regulatory T-cells (Treg) on the adhesion of conventional T-cells (Tconv) to the same DC is quantified by OT-AFM.