JPK Instruments AG - Image Gallery : Force Curves, Maps and Images - AFM

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Nano fibres

Topography and indentation curve, imaged with the NanoWizard® NanoScience AFM

QI™ mode - Quantitative Imaging

for imaging challenging samples with the NanoWizard® 3 AFM family

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NanoWizard® - Combined AFM & Optical Microscopy

Collagen – AFM with phase contrast
AFM topography and optical phase contrast image of collagen.
CHO cells with Hemagglutinin-YFP labeling
DirectOverlay™ of CHO cells with Hemagglutinin-YFP labeling imaged by AFM and confocal microscopy
Hexaphenyl nanofibers with fluorescence
AFM and fluorescence images of p-Hexaphenyl polymer nanofibers
Bacteria from waste water
AFM and optical phase contrast images of filamentous bacteria from waste water
SAOS cells confocal
AFM and confocal fluorescence images of two SAOS cells
Fluorescent polymer spheres
AFM and fluorescence images of 120 nm polymer spheres
MDCK cells confocal
AFM and confocal fluorescence images of MDCK cells with labelled actin
Lipid bilayer confocal
AFM and confocal fluorescence images of a phase separated lipid bilayer sample
Fixed fibroblast with phase contrast
AFM and optical phase contrast images of a fixed fibroblast cell.
50nm beads – AFM with fluorescence
50nm Fluoresbrite™ microspheres YG (polysciences inc.), overlay of the AFM image with corresponding calibrated optical image
SAOS chondrocyte cells – AFM with phase contrast
Screenshot of 50µm AFM scan over phase contrast image
REF52 cells – AFM with fluorescence (incl. screenshot)
REF52 fibroblasts expressing YFP-paxillin - screenshot, height/deflection image
Fixed cell – AFM with DIC
DIC/height/deflection image of a fixed cell
Ptk2 cells – AFM with phase contrast and fluorescence
Images of fixed Ptk2 cells
Living fibroblast cells – AFM with phase contrast and fluorescence
Images of living REF52 fibroblast cells, expressing GFP-paxillin, imaged in buffer, at 37 degrees
Streptococcus bacteria – AFM with DIC
DIC/Height image of streptococcus bacteria adsorbed onto glass, imaged in buffer
Human chromosomes – AFM with DIC
DIC/Height image of human lymphocyte metaphase chromosomes on microscopy slide
Celltraces – AFM with IRM
IRM/Height image of mouse fibroblast cell traces in buffered solution
Cell traces – AFM with TIRF
TIRF/Height image of cell traces, imaged in buffer
Microtubules – AFM with fluorescence
Fluorescence image of microtubules, imaged in buffer
MX1 cell – AFM with phase contrast
An overlay of an AFM image with the corresponding, calibrated optical image
REF52 cells – AFM with fluorescence
An overlay of a 3D contact mode AFM image with the corresponding, calibrated optical image

NanoWizard® - Electrical AFM Measurements

Conductive AFM of a graphite-clay blend
Conductivity landscape and height image of a graphite-clay blend
Domain visualization in lithium niobate crystal
Piezo-response phase, height and amplitude images of lithium niobate crystal
Kelvin probe microscopy (KPM) of lipid film
KPM and height images of lipid film, prepared on mica.

NanoWizard® - Force Curves & Mapping

NanoWizard® - Life Sciences

Pea starch granules
Topography/phase images and zoom steps of pea starch granules
Collagen fibres
Phase and topography images of bundled collagen fibres
Malaria infected red blood cells
3D image of malaria infected red blood cells
Fibrillin microfibrils
Height image and zoom step (height, phase and 3D image) of fibrillin microfibrils
Glucagon
Height images of Glucagon fibres imaged in buffer
E. Coli bacteria in buffer
3D topography, phase and amplitude images of E. Coli bacteria in buffer
OmpF protein crystal
Protein crystal of OmpF (outer membrane porin F), with correlation average image
Xanthan molecules on mica
Height images of Xanthan molecules on mica
Single bacterium (waste water)
Height, amplitude and phase images of a single bacterium from a waste water sample
Thiobacteria on sulphur
Colony of thiobacteria grown on sulphur
Living CHO cell
Height and deflection images of a living CHO (Chinese Hamster Ovary) cell
L929 cell filipodia
Height and deflection images of filipodia between two L929 cells
Cell edge extension (L929)
Cell extension at the edge of a L929 cell
HeLa cells
Topography images of HeLa cells in buffer
DNA molecule on mica
Topography of a DNA molecule on mica
Human lymphocyte chromosomes
3D topography of a group of 3 metaphase chromosomes
PPV viruses zoomed (600nm)
Height/phase/deflection image of PPV viruses, imaged in intermittent contact mode
PPV viruses
Height/phase/deflection image of PPV viruses, imaged in intermittent contact mode
Fe-Pt nanoparticle clusters in lipid bilayer
Height/phase/amplitude image of Fe-Pt nanoparticles embedded in SLPC lipid bilayers
Fe-Pt nanoparticles in lipid bilayer
Height/3D view/phase image of Fe-Pt nanoparticles (6 nm diameter) embedded in SLPC lipid bilayers
Moth's eye
Height image (3D plot), zoom step and corresponding optical microscope image of a moth's eye
Hollow microcapsules
Height images (2 samples) of hollow microcapsules (PSS/PAH), imaged in air
Mammary carcinoma cells
Error signal image of mammary carcinoma cells
Polyelectrolyte shell
Height/DIC image of a polyelectrolyte shell, intermittent contact mode
Ptk2 cells
Height/phase contrast/fluorescence image of fixed Ptk2 cells, imaged in contact mode
Living fibroblast cell
Phase contrast/height/deflection image and zoom steps of living REF52 fibroblast cells
Unfixed collagen in buffer
Height image of unfixed collagen in buffer, showing the 67nm D-repeat
Phase-separated lipid bilayer
Height/phase image of phase-separated lipid bilayer (DOPC/SM/cholesterol)
Ethinylestradiol on glass
Height/phase/amplitude image of ethinylestradiol, prepared on glass
MX-1 cells in buffer solution
Height/phase contrast/amplitude image of a mx-1 cell in buffer solution
TTR amyloid fibres
Height/phase/deflection image of TTR amyloid fibres
Melanocytes
Height/deflection/zoomed image of human melanocytes (rectangled region in the deflection image above) fixed with glutaraldehyde
Implant
Height/deflection/phase image of an implant
Endothelial cells
Height image and 2 zoom steps of endothelial cells connecting via lamellipodia
Osteoblast cells with neuron
Height/deflection image of osteoblast cells with connected neuron
Living endothelial cells in buffer
Height/deflection image of living endothelial cells in buffer solution, contact mode
Blood cell with influenza viruses
Height image and zoomed height image of a red blood cell with influenza viruses, imaged in buffer
Bacterial cell (H. pylori)
Height image in air of a bacterial cell (H. pylori)
Moth wing scale
Height image and corresponding optical microscope image of a moth wing scale
Collagen in air
Height/deflection image of collagen in air
Lipid vesicles
Height/deflection image of lipid vesicles imaged in intermittent contact mode
Lambda-phage DNA in buffer
Height image and zoomed height image of lambda-phage DNA in buffer
Collagen type I micro-fibrils
Height/phase image of collagen type I micro-fibrils on glass
Bacteriorhodopsin patch in buffer
Height/deflection image of a bacteriorhodopsin patch on mica, imaged in buffer
Patterned PDMS polymer
Height/phase image of patterned PDMS polymer
Living fibroblast cell
Height/deflection image of living fibroblast cells, taken in contact mode in PBS
Cytochrome-C proteins
Height image of Cytochrome-C proteins adsorbed onto cover slip inserted into JPK cover slip holder
Bacteriorhodopsin in buffer
Height image of bacteriorhodopsin imaged in buffer solution
DNA fragments
Height image of DNA fragments
Phase-separated lipid bilayer
Height image of phase-separated lipid bilayer (DOPC/SM/cholesterol)
DNA in fluid
Height image of DNA in fluid
Chromosome
Height image of a chromosome imaged in buffer
Cellulose
Height image of bacterial cellulose
Tubulin
Height image of Tubulin molecules forming microtubes (imaged on glass substrate)
Living neuron cell on chip
Height image (3D plot) of a living neuron branch, imaged on a micro-chip
Hemp (bast fibre)
Height image of hemp (Felina) cross section of a bast fibre
DNA molecules
Lambda phage DNA molecules on mica
Sperm
Deflection images of sperm attached to glass with poly-L-lysine and fixed with glutaraldehyde (2%) imaged in PBS
Plasmidic DNA
Plasmidic DNA, adsorbed on mica from Ni2+ containing buffer
Xanthan
Height image of Xanthan
Erythrocytes
Height image of erythrocytes
Hair (topography)
Height image of human hair
Hair (friction)
Friction force image of human hair
Cutinase enzymes
Height image of cutinase enzymes adsorbed to Si substrate
HPI layer of D. radiodurans
Height image of the hexagonally packed intermediate (HPI) layer of D. radiodurans
Barnacle cement
Height image of a network of fibres seen in a sample of protein-based cement produced by barnacles, which is able to stick to many different materia...
DNA-nucleosome complexes
Height image of DNA-nucleosome complexes, imaged in AC mode

NanoWizard® - Nano Sciences, Polymers & Thin Films

Nano fibres
Topography and indentation curve of nano fibres
Zeolite crystal
Height, amplitude and 3D topography of a zeolite crystal
Hexaphenyl nanofibers
Polymer nanofibers formed from p-Hexaphenyl molecules on mica
Dendrimer molecules
Height and phase images of molecules of positively charged dendronized polymer of 3rd generation deposited on mica
Dendronized polymer molecule
Height image of a molecule of dendronized polymer of 3rd generation deposited on mica
Styrene-butadiene copolymer
Height and phase images of polystyrene-block-polybutadiene diblock copolymer
SB block copolymer
Polystyrene-block-polybutadiene diblock copolymer sample - height and phase
Elastomeric polypropylene - 3D views
Height and phase information overlaid in 3D views of an elastomeric polypropylene sample
Elastomeric polypropylene
Height and phase images of an elastomeric polypropylene sample
Polystyrene surface with a failure crack
Height and phase images of a polystyrene surface with a failure crack.
Polystyrene surface with holes
Polystyrene surface with 1 micron holes.
Polymer nanoparticles
Height image of 25 nm polymer nanoparticles
PHB-PHV lamellae
Images of regrowing PHB-PHV lamellae at 120°C after melting the sample at 150°C
Structured PMMA surface
Height/deflection image with zoom steps of a structured PMMA surface
Anodic aluminum
Height image and 3D plot of an ordered pore array of aluminum
Polystyrene film
Height/phase/amplitude image of a polystyrene film
PHB-PHV spherulites
Height/amplitude/phase image of spherulite structures in a PHB-PHV film
OPA on mica
Height/deflection/lateral force image of self-assembled monolayers of octadecylphosphonic acid on mica
CaCO3 aggregates
Height image of CaCO3-aggregates on COOH-terminated gold imaged in air
Block copolymer
Height/phase image of a block copolymer
PHB-PHV copolymer
Height/phase image of thin film of PHB-PHV copolymer
Langmuir blodgett film
Height/Phase image of a Langmuir Blodgett film
Alpha-gel
Height/friction force image of alpha-gel (vibration-damping material)
Teflon
Height/friction force image of Teflon
Polymer blend
Height/phase image of a polymer blend imaged in air
Polymeric micelle aggregate
Height image of a polymeric micelle aggregate and individual micelles
Biaxially-oriented BOPP film
Height/phase image of biaxially-oriented polypropylene (BOPP) film in air
Gelatin-based nanoparticles
Height/phase image of gelatin-based nanoparticles, imaged in air
Thin polymer film
Height image of a thin polymer film on glas, imaged in air
Glassy polymer with inclusions
Height/phase image of a glassy polymer with elastomeric inclusions, imaged in fluid
Polymer blend
Height/amplitude image of a polymer blend imaged in air
Gold nanospheres
Height/Phase image of gold nanospheres
Silicon grid 3µm
2D scan and 3D plot of a silicon grid with 3µm period
Polymer beads with quantum dots
Height image and zoomed height image of polymer beads covered with quantum dots
PHB-PHV spherulites
Height/amplitude image of spherulite structures in a PHB-PHV film
SBS triblock copolymer
Phase/height image of SBS triblock copolymer (Styrene-butadiene-styrene)
J-aggregates
Height image of J-aggregate molecules
Polymer film
Height image of a thin polymer film imaged in air
Ultra-elastic foil
Height image of a stretched ultra-elastic foil
ITO on glass
Height image of ITO on glass
Quantum dots
Height image of quantum dots on silicon surface
Polymer surface with holes
Height image of a polymer surface with holes
Printer paper
Height image of glossy photo paper (for printer)
Polystyrene beads
Height image of polystyrene beads (FluoSphere, 0.2µm diameter)
Ceramic
Height image of ceramic material for cell growth
Fe-Pt nanoparticles
Height image of Fe-Pt nanoparticles (6 nm diameter)
Amphiphilic organic molecules
Height image of self-assembled amphiphilic organic molecules
Atomic mica
Atomic lattice resolution image of mica imaged in contact mode with the standard NanoWizard® AFM mounted on a Zeiss Axiovert 200 inverted light mic...
Spherulitic copolymer
Height image of spherulitic copolymer in thin film on cover slip
Silicon grid 2µm
Height image of a silicon grid with 2 µm period (standard silicon test grid)
PAA on gold
Height image of a single polyacrylamide molecule covalently immobilized onto a gold surface
Gold clusters
Height image of gold clusters imaged in water

NanoWizard® - Nanomanipulation & Lithography

Polymer nanoparticle manipulation
Manipulation of 25 nm polymer nanoparticles
Quantum dots manipulation
Quantum dots (5nm CdSe quantum dots) on glass coverslips
Nano tubes manipulation
Carbon nanotubes on silica surface
Nano wire manipulation
Nano wire manipulation on polymer film
Polymer sphere manipulation
120nm diameter polymer spheres, manipulated in air
Manipulation of a chromosome
Height images of the same chromosome before and after manipulation
Lithography on polymer
Lithography on polymer surface, performed with a constant force of approximately 20 nN: height image, phase image and the template
Lithography on polymer
Height image of the Berlin Bear engraved into a thin polymer film with 6.0 nN force
Collagen manipulation
Collagen type I microfibrils formed on mica

BioMAT™ - Combined AFM & Optical Microscopy

Bacteria on pyrite surface
Images of isolated acidithiobacillus bacteria grown on the surface of pyrite

BioMAT™ - Life Sciences

Bacteria on pyrite surface
AFM deflection signal image and topography image of isolated acidithiobacillus bacteria grown on the surface of pyrite.

TAO™ - Nano Sciences, Polymers & Thin Films

Sample-scanning confocal imaging of quantum dots (2)
AFM height image, fluorescene signal and color-coded AFM height image of quantum dots on a glass coverslip surface
Sample-scanning confocal imaging of quantum dots (1)
AFM height image and optical fluorescene signal of a cluster of 3 quantum dots
Reflection image of AFM tip scanned through a laser beam
Location of the AFM tip in the laser focus for a TAO (Tip Assisted Optics) experiment