Using the DirectOverlay™ software feature

REF52 cells - AFM with fluorescence

Software screenshot shows AFM image recording of a living REF52 fibroblast cell expressing YFP-paxillin.

The optical fluorescence image (green) is in the background. Screen shot of 20µm AFM scan over fluorescent image (63x oil immersion) in SPM software. JPK supports different CCD cameras in the software.
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