Using the DirectOverlay™ software feature
REF52 cells - 3D with fluorescence
Living REF52 fibroblasts expressing YFP-paxillin. An overlay of a 3D AFM image (in red) with the corresponding, calibrated optical image (yellow-green fluorescence).
Fluorescent imaging with 63x oil immersion lens
• Scan field: 100µm * 100µm
• Z-range: 0-3µm