Using the DirectOverlay™ software feature

REF52 cells - 3D with fluorescence

Living REF52 fibroblasts expressing YFP-paxillin. An overlay of a 3D AFM image (in red) with the corresponding, calibrated optical image (yellow-green fluorescence).


Fluorescent imaging with 63x oil immersion lens

• Scan field: 100µm * 100µm
• Z-range: 0-3µm

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