JPK Service Center
NanoWizard® II - Combined AFM & Optical Microscopy
50nm beads – AFM with fluorescence
Overlay of AFM image
50 nm Fluoresbrite™ microspheres YG (polysciences inc.)
Overlay of the AFM image (intermittent contact mode in air) with the corresponding, calibrated optical image (epifluorescence using 63x oil immersion lense)
• Scan field: 20µm * 20µm
• Z-range: 0-50nm
Calibrated fluorescence image
Overlay of the AFM image with the corresponding calibrated fluorescence image: See also the technical report jpk-tech-optical-integration.pdf for more detailsUncalibrated fluorescence image
Overlay of the AFM image with the corresponding uncalibrated fluorescence image: The uncalibrated image is shifted with respect to the AFM image (see arrows). See also the technical report jpk-tech-optical-integration.pdf for more details


